75:T4e9,Epitalon, a synthetic tetrapeptide (Ala-Glu-Asp-Gly), has shown promising effects on longevity in several studies: 1. **Lifespan extension**: Epitalon increased the mean lifespan of mice by 13.5% and the maximum lifespan by 13.9% [1, 2]. In Drosophila melanogaster, it increased lifespan by 11-16% [3]. 2. **Telomere elongation**: Epitalon induced telomerase activity and telomere elongation in human fibroblasts, allowing cells to make 10 extra divisions beyond the Hayflick limit [4]. 3. **Antioxidant effects**: It reduced intracellular reactive oxygen species in oocytes [5] and inhibited free radical processes in rats and D. melanogaster [6]. 4. **Gene expression**: Epitalon activated chromatin in lymphocytes of older individuals [7] and reactivated ribosomal genes [8]. 5. **Melatonin regulation**: It increased night melatonin levels in old rhesus monkeys [9]. 6. **Tumor suppression**: Epitalon inhibited spontaneous tumor development in mice [10, 11]. 7. **Mortality reduction**: It decreased mortality rates by 52% in D. melanogaster and rats, and by 27% in C3H/Sn mice [6]. These findings suggest that Epitalon may promote longevity through multiple mechanisms, including antioxidant effects, telomere maintenance, and gene regulation.76:T453,Female transgenic FVB/N mice carrying the breast cancer gene HER-2/neu received epithalon (Ala-Glu-Asp-Gly) in a dose of 1 mg subcutaneously 5 times a week to from the 2nd month of life to death. Epithalon prolonged the average and maximum lifetimes of mice by 13.5 (p<0.05) and 13.9%, respectively. The peptide prolonged the average lifetime of animals without neoplasms (by 34.2%, p<0.05). Epithalon decelerated the development of age-related disturbances in reproductive activity and suppressed the formation of neoplasms. The peptide decreased the incidence of breast adenocarcinomas, lungs metastases (by 1.6 times, p<0.05), and multiple tumors (by 2 times). Epithalon 3.7-fold increased the number of mice without breast tumors (p<0.05), while the number of animals with 6 or more breast tumors decreased by 3 times (p<0.05). Epithalon prolonged the lifetime of mice with breast tumors by 1.4 times (p<0.05). These results indicate that Epithalon possesses geroprotective activity and inhibits breast carcinogenesis in transgenic mice, which is probably related to suppression of HER-2/neu expression.77:T453,Female transgenic FVB/N mice carrying the breast cancer gene HER-2/neu received epithalon (Ala-Glu-Asp-Gly) in a dose of 1 mg subcutaneously 5 times a week to from the 2nd month of life to death. Epithalon prolonged the average and maximum lifetimes of mice by 13.5 (p<0.05) and 13.9%, respectively. The peptide prolonged the average lifetime of animals without neoplasms (by 34.2%, p<0.05). Epithalon decelerated the development of age-related disturbances in reproductive activity and suppressed the formation of neoplasms. The peptide decreased the incidence of breast adenocarcinomas, lungs metastases (by 1.6 times, p<0.05), and multiple tumors (by 2 times). Epithalon 3.7-fold increased the number of mice without breast tumors (p<0.05), while the number of animals with 6 or more breast tumors decreased by 3 times (p<0.05). Epithalon prolonged the lifetime of mice with breast tumors by 1.4 times (p<0.05). These results indicate that Epithalon possesses geroprotective activity and inhibits breast carcinogenesis in transgenic mice, which is probably related to suppression of HER-2/neu expression.78:T469,The developmental potential of oocytes decreases with time after ovulation in vivo or in vitro. Epitalon is a synthetic short peptide made of four amino acids (alanine, glutamic acid, aspartic acid, and glycine), based on a natural peptide called epithalamion extracted from the pineal gland. It is a potent antioxidant, comparable to melatonin, that may confer longevity benefits. The current study aims to test the protective effects of Epitalon on the quality of post-ovulatory aging oocytes. Epitalon at 0.1mM was added to the culture medium, and the quality of oocytes was evaluated at 6h